Nanobacteria: History and References

Scientific journalism at it best provides a detailed analysis of a topic that appeals to both a scientist and a layperson. It supports the case with detailed references to the scientific literature and allows the reader to research the issue independently. The best scientific journalism I read in 2004 was

and the best candidate for 2005 is

In both cases, the authors exposed systematic refusal to see the evidence, a common phenomenon in medicine which my favorite cardiologist colleague calls the “cognitive gap.” Of course it is sometimes systematic suppression of evidence for personal or corporate gain as in the case of deaths caused by VIOXX or contaminated polio vaccine. In the case of nanobacteria, it appears to be mostly the cognitive gap since there are some scientists that claim nanobacteria do not exist even after reviewing the evidence.

The interesting thing about both nanobacteria and SV40 is that they increase the risk of both heart disease and cancer in over 100 million Americans. Future research may demonstrate that they cause more deaths than the current known single causes of death (cigarettes for number of people killed, alcohol for number of years of life extinguished). Right now they are both in the “cognitive gap” stage where the medical community is like primitive tribesman brought to New York in the early years of America. When standing right beside a large ocean-going ship, they could not see it because it did not fit into their frame of reference.

But I digress. What is interesting about The Calcium Bomb: The Nanobacteria Link to Heart Disease & Cancer? One example is that Dr. E. Olavi Kajander discovered nanobacteria while working in 1985 as a postdoctoral research fellow in the laboratories of Professor Dennis Carson at the Scripps Research Institute near San Diego, California. In 1987, Kajander returned to Finland to establish his own cell culturing lab at the Biochemistry and Biotechnology Department of the University of Kuopio where he demonstrated that nanobacteria were coming from contaminated fetal bovine serum. Since bovine serum is used to make many of our vaccines, almost anyone who has been vaccinated is infected with nanobacteria.

In 1998, Kajander applied for a patent which was subsequently granted by European and American patent authorities and now forms the basis of conventional treatment. Investors may want to take a look at the prospectus of Nanobac Life Sciences, Inc.

United States Patent 5,135,851, Kajander, August 4, 1992

Culture and detection method for sterile-filterable autonomously replicating biological particles

Abstract: Novel autonomously replicating biological particles resembling bacteria and having most surprising properties were discovered from cell culture sera and other biological samples alleged to be sterile according to the current testing methods. These slowly growing agents named Nanobacteria are smaller than any known cell-walled bacteria. They pass through sterile filters, even with pore sizes smaller than their diameter. They cannot be cultured on any standard microbiological media. With the isolation and detection methods provided here they are commonly detectable in animal or human serum. This patent holds for methods of their culture, detection, purification, and elimination and described the necessary reagents for that. Autonomously replicating particles can be cultured in RPMI 1640, or in DMEM, or in other cell culture media. Optimal growth can be obtained by supplementing the culture medium with 10-20% sterile fetal bovine serum. Addition of small amounts of D,L or L selenomethionine together with nucleotide precursors may improve growth. Culture is started by addition of the test sample to the medium in a cell culture vial which is thereafter incubated under standard mammalian cell culture conditions for at least 15 days. Biological samples are preferably sterile-filtered before culture through 0.22 micron filters. The growth of Nanobacteria, if present, can be seen using microscopy at high magnification. The organisms can be made more visible by DNA staining and immunostaining done either separately or simultaneously to a fixed preparation. Nanobacteria can be cultured without mammalian cells, but co-culture together with an adherent cell line like 3T6 is useful because 3T6 cells can take Nanobacteria inside the cells. This aids the staining of the preparations. Intracellular agents are not lost during fixation and staining. Nanobacterial antigens can be prepared by specific culture, harvest, purification and solubilization methods. Immunization of rabbits with the solubilized antigen (treatment with proteinase K and with 1 N HCI) produces highly specific antibodies to Nanobacteria. Gamma-irradiation of culture serum at 2.5-4.0 megarads, preferably in addition with treatment using solid-phase bound antibodies enables preparation of Nanobacteria-free serum. Use of this serum creates sterile culture medium for the culture and detection of Nanobacteria. Double staining combining Hoechst No. 33258 stain and immunofluorescence specifically distinguishes Nanobacteria from other cell culture contaminants.

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