Mycoplasma Fementans Incognitus Frequencies Version 1.0

United States Patent	5,242,820
Lo	September 7, 1993

Pathogenic Mycoplasma (Mycoplasma Fermentans Incognitus)

AbstractThe invention relates to a novel pathogenic mycoplasma isolated from patients with Acquired Immune Deficiency Syndrome (AIDS) and its use in detecting antibodies in sera of AIDS patients, patients with AIDS-related complex (ARC) or patients dying of diseases and symptoms resembling AIDS diseases. The invention further relates to specific DNA sequences, antibodies against the pathogenic mycoplasma, and their use in detecting DNA or antigens of the pathogenic mycoplasma or other genetically and serologically closely related mycoplasmas in infected tissue of patients with AIDS or ARC or patients dying of symptoms resembling AIDS diseases. The invention still further relates to a variety of different forms of vaccine against mycoplasma infection in humans and/or animals.

Inventors:	Lo; Shyh-Ching (Potomac, MD)
Assignee:	American Registry of Pathology (Washington, DC)
Family ID:	27401851
Appl. No.:	07/710,361
Filed:	June 6, 1991

Related U.S. Patent Documents


Application Number	Filing Date	Patent Number	Issue Date
*265920*	Nov 2, 1988
875535	Jun 18, 1986

Other References

Marquart et al (1985) Mycoplasma-Like Structures . . . Eur J Clin Microbiol 4(1):73-74. .
Lo et al (1989) A Novel Virus-like Infectious Agent . . . Am J Trop Med Hyg 40(2):213-226. .
Lo et al (1989) Identification of M Incognitus . . . Am. J. Trop-Med. Hyg 41(5):601-616. .
Lo et al (1989) Association of the Virus-like Agent . . . Am J Trop Med Hyg 41(3):364-376. .
Lo et al (1989) Fatal Infection of Silvered Leaf Monkeys . . . Am. T Trop Med Hyg 40(4):399-409. .
Lo et al (1989) Virus-like Infectious Agent . . . Am J Trop Med Hyg 41(5):586-600. .
Marquart et al (Feb. 1985) Abstract Only Eur J Clin Microbiol 4(1):73-74. .
Hu et al (1990) Gene 93:67-72..

Primary Examiner: Nucker; Christine M.
Assistant Examiner: Preston; D. R.
Attorney, Agent or Firm: Venable, Baetjer, Howard & Civiletti

Government Interests
The invention described herein was made in the course of work under a grant or award from the United States Department of the Army.

Parent Case Text
CROSS-REFERENCE TO RELATED APPLICATIONSThis is a continuation-in-part of U.S. patent application Ser. No. 265,920, filed Nov. 2, 1988, now abandoned, which is a continuation-in-part of U.S. patent application Ser. No. 875,535, filed Jun. 18, 1986, now abandoned.

Claims
What is claimed is:1. A biologically pure mycoplasma isolated from tissues of patients with AIDS comprising the mycoplasma produced by the cell line ATCC No. CRL 9127.
2. A biologically pure mycoplasma having the identifying characteristics of M. fermentans incognitus, ATCC 53949.

Description
BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to a novel strain of mycoplasma isolated from a patient with AIDS. The mycoplasma is closely related to a species of human mycoplasma, M. fermentans. Upon characterization of this mycoplasma, it may be classified as a unique strain within the species M. fermentans incognitus. This novel strain of nycoplasma is referred to hereinafter as the incognitus strain or M. fermentans incognitus.

The invention also relates to use of the mycoplasma M. fermentans incognitus as well as all strains of M. fermentans in detecting specific antibodies in sera of patients with AIDS or an acute fulminant systemic disease and/or animals and its use as a vaccine against infection by the mycoplasma. The invention further relates to incognitus strain-specific antibodies and cross-reactive which later break up into individual cells that are capable of passing through membrane filters of pore size 0.45 .mu.m or even 0.22 .mu.m.

Those with Lyme disease may already be aware of this coinfection. It appears to be circulating with the 2017 flu causing extended periods of serious illness. Frequencies are available to subscribers.

Lyme Disease Persisters – Borrelia

The Biofilm frequency sets include many strains of borrelia which tend to cause increased blood pressure. Heat (hot bath 119 degrees or infrared lamp) dropped blood pressure to normal. Further investigator indicated that borrelia biofilms were disabled by heat and frequency sets were developed to target these organisms. Repeated application of biofilm frequencies normalized blood pressure for short periods. Only with application of one of the antibiotics in the second paper below did blood pressure normalize for extended periods.

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Lyme Disease May Linger for 1 in 5 Because of “Persisters”

By Melinda Wenner Moyer in Scientific American on September 1, 2015

Lyme disease is a truly intractable puzzle. Scientists used to consider the tick-borne infection easy to conquer: patients, diagnosed by their bull’s-eye rash, could be cured with a weeks-long course of antibiotics. But in recent decades the U.S. Centers for Disease Control and Prevention has realized that up to one in five Lyme patients exhibits persistent debilitating symptoms such as fatigue and pain, known as post-treatment Lyme disease syndrome, and no one understands why. The problem is growing. The incidence of Lyme in the U.S. has increased by about 70 percent over the past decade. Today experts estimate that at least 300,000 people in the U.S. are infected every year; in areas in the Northeast, more than half of adult black-legged ticks carry the Lyme bacterial spirochete, Borrelia burgdorferi. Although the issue is far from settled, new research lends support to the controversial notion that the disease lingers because these bacteria evade antibiotics—and that timing drug treatments differently could eliminate some persistent infections…

Identification of novel activity against Borrelia burgdorferi persisters using an FDA approved drug library. Jie Feng, Ting Wang, Wanliang Shi, Shuo Zhang, David Sullivan, Paul G Auwaerter and Ying Zhang. Emerging Microbes and Infections (2014) 3, e49; doi:10.1038/emi.2014.53; published online 2 July 2014

Although antibiotic treatment for Lyme disease is effective in the majority of cases, especially during the early phase of the disease, a minority of patients suffer from post-treatment Lyme disease syndrome (PTLDS). It is unclear what mechanisms drive this problem, and although slow or ineffective killing of Borrelia burgdorferi has been suggested as an explanation, there is a lack of evidence that viable organisms are present in PTLDS. Although not a clinical surrogate, insight may be gained by examining stationary-phase in vitro Borrelia burgdorferi persisters that survive treatment with the antibiotics doxycycline and amoxicillin. To identify drug candidates that can eliminate B. burgdorferi persisters more effectively, we screened an Food and Drug Administration (FDA)-approved drug library consisting of 1524 compounds against stationary-phase B. burgdorferi by using a newly developed high throughput SYBR Green I/ propidium iodide (PI) assay. We identified 165 agents approved for use in other disease conditions that had more activity than doxycycline and amoxicillin against B. burgdorferi persisters. The top 27 drug candidates from the 165 hits were confirmed to have higher anti-persister activity than the current frontline antibiotics. Among the top 27 confirmed drug candidates from the 165 hits, daptomycin, clofazimine, carbomycin, sulfa drugs (e.g., sulfamethoxazole), and certain cephalosporins (e.g. cefoperazone) had the highest anti-persister activity. In addition, some drug candidates, such as daptomycin and clofazimine (which had the highest activity against non-growing persisters), had relatively poor activity or a high minimal inhibitory concentration (MIC) against growing B. burgdorferi. Our findings may have implications for the development of a more effective treatment for Lyme disease and for the relief of long-term symptoms that afflict some Lyme disease patients.

Mosquitos and the West Nile Virus Version 3.0

Madeline Drexler does a fascinating piece of work in her book, Secret Agents, on the outbreak of the West Nile Virus in New York in 1999 where it tooks weeks to find out what virus was killing birds and people. Finally, on 22 September 1999, a CDC scientist loaded samples of the virus genome into a machine that automatically sequenced the DNA strands. The next morning, he submitted strings of genetic code over the internet to the National Library of Medicine GenBank and got a hit on the West Nile Virus.

Frequencies of pathogens are related to DNA. It is a lot faster and easier to determine a frequency sequence and then search gigabytes of data in the Frequency Research Foundation database than it is to spend weeks trying to culture a virus. A pathogen can often be identified within a few minutes.

After returning from long European trip in June 2016, I woke up at night with a pain in my chest. Testing for frequencies, then scanning the database, showed I was infected with the West Nile Virus. I then tested my dog who was sleeping with me and he had obviously picked up the virus in the Massachusetts woods while I was away.

Frequencies for several strains of West Nile Virus needed to be run to clear up the dog and me. All strains with their latest updates are posted on the Frequency Foundation subscribers site.

In addition, frequencies were transmitted to eliminate mosquitos from the woods where he stayed while I was gone using the Frequency Foundation mosquito service which requires only a small fee per month.

Mosquitos transmit many pathogens that have been found in my area:

Lyme disease
Zika virus
West Nile Virus
etc.

A few years ago my wife picked up a shingles infection from a mosquito bite on a houseboat in Amsterdam. I was bitten by many malaria infested mosquitos in India earlier this year. Getting rid of the mosquitos is a lot less painful than dealing with the infections.

Our research shows that every mosquito species transmits a signature pathogen into the human body and targeting that organism in the mosquito causes the mosquito to die. Our database has frequencies for hundreds of species of mosquitos, ticks, and flies gathered over the last decade with the help of many clients and fellow researchers.

Apparently, once you are infected with a mosquito’s signature pathogen it stays there for life unless removed with frequencies. Then you become a tender morsel for mosquitos with that frequency sequence. This explains why mosquitos love specific individuals.

USGS CDC West Nile Virus 8 Aug 2014

While there is only animal West Nile Virus activity reported in Massachusetts, a client picked up a strain in Whole Foods in 2014 so there are unreported human cases. West Nile is a nasty virus so it is good to have updated frequencies on hand.

Mosquito’s transmitting West Nile Virus can be found all over the United States. This virus has been part of the lyme series since 2005 and was an important component of the swine flu which distributed it everywhere.

According to the Mayo Clinic, humans can become infected with West Nile after being bitten by an infected mosquito. Mosquitoes contract the virus when they feed on infected birds. While there have been no reported human cases yet, officials recommend taking the necessary precautions to prevent the virus – such as using insect repellent outdoors, especially from dusk until dawn, and wearing long pants and long sleeved shirts.

People should also make sure their door and window screens are not broken, health officials said; and to stop mosquitoes from breeding, they should empty any standing water that might have collected in garden pots, pools or trash.

Frequency Research Foundation Mosquito Research Program

For the past decade fellow researchers have worked with the Frequency Research Foundation to identify frequencies that will make over 150 species of mosquitos go away, including those mosquitos carrying the West Nile Virus.

Specific areas can be targeted for a small monthly fee. Contact [email protected]

Previous West Nile Virus Experience

A client fell down three times in one week incurring some serious scrapes and brushes. Photoanalysis showed she had mosquito frequencies. The specific species of mosquito was targeted for removal of material injected by the mosquito and removal of the mosquito species from the area around her home.

A hadoscan showed low energy spots in her body from the mosquito frequencies and particularly along her spine. West Nile Virus can become a neurological infection which would explain the falling episodes.

Following application of the mosquito frequencies, an unidentified virus in the Lyme program set was discovered. Suspecting that this might be West Nile Virus, an electron microscope photo of the virus was analysed. An exact match was confirmed.

Another client thought he was dying. Application of these frequencies for 12 hours brought him back to normal.

Stanford Magazine – New Tick Organism/Lyme in California

PESTS POSE DOUBLE THREAT

In the spring, a young man’s fancy turns to thoughts of love; a young tick’s to thoughts of lunch. (Which, should he happen to be an outdoorsy type, could well be that young man.) Prodigious in wooded and grassy areas and in sandy soil near rivers, ticks carry several pathogens that can be transmitted to humans via their bite. The best-known isBorrelia burgdorferi, the microorganism that causes Lyme disease, which initially presents as flu-like symptoms and, left untreated, can cause persistent joint pain, cognitive deficits and, in rare cases, cardiac arrhythmia. But there are others. In 2010, the National Academy of Sciences Institute of Medicine called ticks “the Swiss Army knife of disease vectors.”

In a study published in March in the journal Emerging Infectious Disease, Dan Salkeld, a disease ecology research associate at the Woods Institute for the Environment, encountered a newly identified human pathogen, Borrelia miyamotoi, along with B. burgdorferi, in nearly all the Bay Area recreation areas he and his collaborators examined. B. miyamotoi had previously been known to infect ticks, but it was only in 2013 that the first human cases in the United States were reported, and those were in New England. Given that little is known about the transmissibility of B. miyamotoi from ticks to humans and its health consequences, it’s possible that other cases may have gone undiagnosed.

Stanford students taking the Conservation Medicine in Practice course taught by Salkeld and Woods senior fellow Eric Lambin in the spring of 2012 aided the research effort by collecting tick samples from the Jasper Ridge Biological Preserve and neighboring communities. Salkeld then expanded the study to include a total of 12 locations in Santa Cruz, Santa Clara, San Mateo, Marin, Mendocino and Contra Costa counties. Across all sites, 3.6 percent of the ticks were infected with Borrelia species, a relatively low prevalence compared with Northeastern states. Still, the Lyme-causing variant, B. burgdorferi, was detected at four sites, while B. miyamotoi was found at seven sites.

The surprising findings are “an important step toward dispelling the perception that you cannot acquire Lyme disease in California,” says Ana Thompson, executive director of the Bay Area Lyme Foundation, which funded the research.See also:

Human Borrelia miyamotoi Infection in the United States

N Engl J Med 2013; 368:291-293January 17, 2013DOI: 10.1056/NEJMc1215469

Article
Citing Articles (16): To the Editor:
Borrelia miyamotoi, a spirochete that is genetically related to the species of borrelia that cause relapsing fever, has been detected in all tick species that are vectors of Lyme disease.1,2 It was detected in Ixodes scapularis ticks from Connecticut in 2001 and subsequently has been detected in all areas of the United States where Lyme disease is endemic. The first human cases of B. miyamotoi infection were reported in Russia in 2011.3 We now provide evidence of B. miyamotoiinfection and the prevalence of this infection among people in the United States.

Lyme: It’s not what your doctor is telling you it is!

Lyme Cover Up Rages On

Submitted by Lois Rain on July 1, 2011 – 9:16 pm

What have you heard about tickborne Lyme for the last 30 years? That it’s rare, scary, acute, treatable… The government warns about its spread and implores people to go to a doctor upon seeing the telltale bulls-eye rash. What happens when they actually do?

Many have researched the controversial beginnings of Lyme disease, but this article focuses on what happens to victims when they contract it and what they can do now.

Under Our Skin is a documentary that lends a voice to the many who in fact suffer from chronic Lyme and are victims of a greater abomination.

Why do conventional doctors tell them it’s in their head? Why won’t they quickly test for it? Treat it? Acknowledge it? Why do medical boards shut down doctors who can treat and cure Lyme?

Lyme’s Disease is NOT RARE – Bigger Than AIDS

In the late 70s, a Lyme, Connecticut mom reported a mysterious new disease sweeping the town, leaving its people with debilitating, chronic symptoms. In 1981, Dr. Willy Burgdorfer discovered the Lyme bacteria, called Borrelia burgdorferi.

The bacteria spirochetes closely resemble syphilis in their make up. While a carrier tick is feeding, its backwash enters the host and transmits Lyme. The corkscrew spirochetes wreak havoc, drilling into any healthy cells and tissue. They create painful, crippling neurological and immunological damage.

In the beginning, doctors only knew that it resembled syphilis but remained unaware of its wide spread, how to proceed, and the political, medical clash that awaited them.

In recent years, the CDC has reported over 35,000 new Lyme cases annually but admit that since it is so overlooked the actual number may be 12 times higher, up to 420,000 cases each year.

Think of how much more likely it is to contract Lyme than the media-touted West Nile virus which is only reported at around 1,300 cases annually. If the actual number of Lyme cases is even just a modest amount above the CDC’s 35,000, then Lyme is far more prevalent than AIDS, reported at 39,000 cases annually.

Since 1982, the number of cases continues to climb and spike prompting media reports and health officials to label it epidemic as early as 1989. Reported cases have tripled since 1992. Every summer we hear the same cautionary reports. Yet doctors constantly tell their patients “You don’t have Lyme” or “Lyme only happens in such-and-such state, not here.”

Lyme is a national health crisis in every state and has traveled the globe! Read more …

Lyme Frequencies – Version 6.2

CDC publishes a map of Lyme disease risk showing the gradual spread of Lyme disease across the United States. CDC estimates reported cases are 10% of actual cases, so Lyme disease is a massive epidemic that has spread to other countries as well. Over 50% of the people I now test are positive for Lyme organisms, although many of them may temporarily be asymptomatic. Merck researchers observe Lyme mycoplasma in over 95% of the population.

Lyme disease is unlike any other syndrome on the planet. Alternative practitioners note that it is more difficult to treat than cancer. The reason for this is that it is a complex of thousands of viruses, parasites, fungi, mycoplasma, and other organisms. There is enough published circumstantial evidence to conclude that it is designed to be undetectable in the early stages, untreatable, and eventually, permanently disabling. It is also gets worse if treatment is attempted, a phenomenon observed in the Herxheimer reactions noted by those who try to treat their Lyme disease. Frequencies need to be carefully designed to avoid these reactions as much as possible.

During 1991-2002, the reported incidence of Lyme disease nearly doubled. Most cases continued to occur in northeastern, mid-atlantic, and north-central states, and the largest population of cases continued to be reported among persons aged 5-9 years and 50-59 years, possibly as a result of greater exposure than other groups to infected ticks, less frequent use of personal protective measures, differential use of health-care services, and/or reporting bias. For additional information, visit CDC’s web site at http://www.cdc.gov/ncidod/dvbid/lyme/index.htm.

Analysis of hundreds of people and animals with Lyme disease have produced the latest Frequency Foundation Lyme Disease Version document which has now grown to a small book of over 100 pages. In the latest version, frequencies are published in ready to run F165 programs which can be modified to run on any device. The frequency set is certainly not finished as new Lyme organisms or updates to older frequencies are discovered on a daily basis.

Note: Current Frequency Foundation subscribers can update these lyme sets by downloading Borrelia biofilm frequency sets.

Lastest Upgrade to Lyme Frequencies

Refinement of frequency sets are based on work with hundreds of people and animals on a daily basis for many years. Several new programs and major updates to previous programs are included in the latest version:

• Lyme heart viruses – a group of Lyme viruses tend to proliferate in the heart and cause pain and palpitations in some individuals.
• Lyme stealth organisms – certain organisms take years to detect and are very persistent. Some of them have crippling effects. These have been packaged into a separate program.
• Lyme insects – funded research on insect frequencies has led to the creation of a Lyme insect program. Most of these frequencies are tick frequencies.

In addition, previous programs contain hundreds of new frequencies.

• Lyme mycoplasma – the core of Lyme disease is a mycoplasma bioengineered from weapons grade brucella at Plum Island laboratories [1].
• Lyme viruses – many of these are crippling. Many of the most dangerous viruses in the world were brought to Plum Island for experimentation, the Rift Valley virus from Africa, for example.
• Lyme babesia – there are hundreds of strains of the babesia parasite.
• Lyme parasites – there are many other parasites, many of which are released after killing babesia
• Lyme cells – the Lyme mycoplasma get inside cells and cause genetic mutation. These aberrated cells often cause pain and some can progress to cancer.
• Lyme bacteria – many of the bacteria in Lyme emit neurotoxins. These are what physicians typically treat with antibiotics. Paradoxically, these are the easiest to eliminate with frequencies. Lyme is a much bigger problem than spirochetes which many have discovered after their physician pronounced them cured.
• Lyme rickettsia – variants of this organism cause Rocky Mountain Spotted Fever and other diseases.
• Lyme fungus – these are very difficult to get rid of without frequencies. Some produce toxins which get into cyclic biochemical pathways that prevent elimination without frequencies or special treatment.
• Proteus mirabilis – another pathogen always found in Lyme.
• Gnathastoma spinigerum – another pathogen always found in Lyme.

While sets of the Lyme frequencies can be manually run on any device, they are best run with programmable frequency generators like the F100 series from Atelier Robin due to the length of the programs. Frequency octaves can be created for any device as described in the documentation.

Lyme Frequency Set Version 4.2

Refinement of frequency sets has continued on a daily basis since Release 4.0. Updating these sets has become a major task and can now only be done in increments. This release has a significantly upgraded Rickettsia program as requests have been received for frequencies for this persistent bacteria that gets into cells.

In addition, a scalar octave table has been added for the Rickettsia frequencies. This will allow finding a frequency in the range of any frequency device without doing calculations. For those having difficulty understanding the F165 programming language, please review the section where an F165 program is translated into frequencies for the FSCAN. This, in combination with the Rickettsia scalar octave table will allow creation of programs for any Rife plasma or pad device. As other programs are upgraded at a future date, scalar octave tables will be provided.

All repeat commands have been removed from programs. They may need to be run multiple times. Symptom patterns or some form of kinaesthesiology must be used to determine whether to run them again (muscle testing or a dowsing technique).

Background on the Lyme Frequency Sets

The dark field microscopic photo shows red blood cells (circles), one of the many strains of Lyme parasite (large white object), brucella (small white spots), and one of the crystalline forms of the Lyme mycoplasma (faint thin lines).

A steady stream of email and voice mail asking for frequencies for various pathogens is received by the Frequency Research Foundation on a daily basis. Because Lyme disease is such a major public health problem, the latest frequency sets are published here for a small fee that covers administrative costs for maintaining them. Periodically, they are updated as research progresses. For those who want regular updates to all frequency sets, a subscription fee for 2006 may be selected below.

These frequencies are for research purposes only and may be helpful, harmful, or ineffective depending on how they are used. They are offered as a service for fellow researchers to experiment with at their own risk. Care must used to avoid herxheimer effects or other negative reactions.

There are many Lyme programs that must be used in combination based on findings from research on hundreds of humans and animals in the U.S., Europe, Ukraine, and Russia by multiple researchers who pool their experience. It is a tedious and demanding process to tease apart the frequencies for various organisms, particularly when some of them overlap. Therefore, the frequencies are constantly upgraded based on new research findings. For some background on evolution of these frequencies see: https://www.frequencyfoundation.com/2005/07/update-on-lyme-its-not-just-in-ticks.html

The programs are recommended to be run in the following order:

Mycoplasma/Brucella/Visna virus
Parasites
Bacteria
Cells
Viruses
Proteus mirabilis
Gnathastoma spinigerum
Rickettsia

Programs are specified in the F100 programming language which is documented at http://www.atelierrobin.net. This is a powerful scripting language for Rife frequencies as it allows careful control of dwell, pulse, and duty cycles. All programs run square waves with a duty cycle of 66.6% to take advantage of harmonics.

Programs run the primary frequency as a carrier wave modulated by scalar octaves of the primary frequency. Those who want to run these frequencies on devices with a limited frequency range should use the scalar octaves generated by the programs. Scalar octaves and how to calculate them are described at:
https://www.frequencyfoundation.com/2006/09/calculating-scalar-octaves-for-rife.html

By the end of 2008 many upgrades to the Lyme frequencies have been created with thousands of new frequencies. See a later posting or subscribe using the button on the left side of this page to get regular updates.

Human Borrelia miyamotoi Infection in the United States

To the Editor: